Application of Gold Label Card Colloidal Gold Immunochromatographic Technology i

He Wei et al. established a simple and convenient gold labeled card colloidal gold test strip to detect anti schistosomiasis antibodies in livestock serum. 33 positive bovine serum and 50 positive pig serum were detected, with a positive detection rate of 100%. 23 negative bovine serum and 50 negative pig serum were detected, and 1 and 3 positive reactions were found, with a negative coincidence rate of 95.7% and 94.0%, respectively. A total of 16 bovine and sheep serum samples infected with Fasciola hepatica were detected, with 3 positive samples and a cross reaction rate of 18.8%, indicating that this method has high sensitivity and strong specificity. Wang Yanhua et al. used an immunocolloidal gold test strip established to detect antibodies against Toxoplasma gondii in animal serum, and conducted a control experiment using the Toxoplasma gondii IHA diagnostic method. The results showed that the sensitivity of the gold test strip was higher than that of IHA, and the consistency rate with the Toxoplasma gondii IHA detection results was 82.500, and the antibody was detected 2 days earlier than the Toxoplasma gondii IHA diagnostic reagent. Zhang Shoufa et al. used colloidal gold immunochromatography and ELISA to detect 147 serum samples in the field, and found that GICA had a positive conformity rate of 100% and a negative conformity rate of 88.400 compared to ELISA, with a total conformity rate of 87.8%. They believe that compared with ELISA, GICA has the advantages of high sensitivity, strong specificity, accuracy, single operation, large-scale detection, easy to understand results, no need for instruments and equipment, and no need for technical training for operators=“ http://2meiwang.cn "The colloidal gold immunochromatography method, as a serological detection method for bovine Newcastle disease, has good practical value and is suitable for application in grassroots veterinary units."