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    The principle of colloidal gold immunochromatographic technology using gold labe

    Time:2017-01-08Publisher:Xingyuan

    Gold Label Card

    Colloidal gold immunolabeling technology is a new type of immunolabeling technology that uses colloidal gold as a tracer marker to apply to antigens and antibodies. It mainly utilizes the high electron density characteristics of gold particles. At the binding site of gold labels, black brown particles can be seen under the microscope. When these markers aggregate in large quantities at the corresponding ligand, red or pink spots can be seen by the naked eye, so it is used in qualitative or semi quantitative immune detection methods. Immunochromatography (IC) technology is a simple and immunological detection technique established in the early 1980s by combining immune technology and chromatographic chromatography technology." Colloidal Gold Immunoassay (GICA) It is a solid-phase labeling immunoassay technique that combines various methods such as colloidal gold labeling and immunochromatography. Its principle is to use strip like fiber chromatography material as the solid phase, and through capillary action, the sample solution swims on the chromatography strip. At the same time, the analyte in the sample undergoes a highly specific and affinity immune reaction with the receptor (antigen or antibody) of the analyte on the chromatography material. During the chromatography process, the immune complex is enriched or intercepted in the area of the chromatography material (detection band), and a visible marker (colloidal gold) is used to visually observe the experimental phenomenon (coloring), while the free marker crosses the detection band and automatically separates from the binding marker. The Gold Label Card Shell Colloidal Gold Immunoassay (GICA) technique utilizes the colorimetric characteristics of colloidal gold itself in combination with immunoassay technology to achieve detection purposes. During chromatography, colloidal gold is used as a marker, and the complex between the marker and the analyte is captured by the corresponding ligand and concentrated for color development. The presence or absence of color bands on the fiber membrane is used for detection& Nbsp